Coding

Part:BBa_K1779200:Design

Designed by: Xingyang Qiu   Group: iGEM15_CHINA_CD_UESTC   (2015-09-03)


Integration of three genes, including mamW, RFP and laccase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1005
    Illegal AgeI site found at 1117
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed primers with linker will mamW, RFP and laccase three genes linked by PCR of fusion.The mamW and RFP genes'stop codons have been removed.


Source

mamW gene is from Magnetotactic bacteria's genomic sequence. RFP gene is obtained from the iGEM official bio bricks, which numbered BBa_E1010. Laccase gene is obtained from the iGEM official bio bricks, which numbered BBa_K863005.

References